Homogeneous amperometric immunoassay for theophylline in whole blood.
Biosens Bioelectron
; 8(9-10): 415-9, 1993.
Article
in En
| MEDLINE
| ID: mdl-8311938
ABSTRACT
A homogeneous spectrophotometric EMIT immunoassay kit for the quantitation of theophylline in serum or plasma has been modified to produce a rapid, amperometric immunoassay requiring a 50 microliters whole blood sample. The basis of the detection system for the assay is the electrochemical oxidation of NADH produced by G6PDH-labelled theophylline at a potential of +150 mV vs Ag/AgCl using platinised activated carbon (PACE) electrodes. Comparison of the amperometric whole blood method with the conventional spectrophotometric plasma assay produced a reasonable correlation y = 0.90x - 1.01, (r = 0.98, n = 12). The advantage of the new method is that simple and robust instrumentation can rapidly determine theophylline in whole blood with no sample pre-treatment or separation steps.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Theophylline
/
Enzyme Multiplied Immunoassay Technique
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
Biosens Bioelectron
Journal subject:
BIOTECNOLOGIA
Year:
1993
Document type:
Article
Affiliation country:
United kingdom