A double sandwich monoclonal enzyme immunoassay for detection of hepatitis B surface antigen.
J Immunoassay
; 14(1-2): 21-31, 1993.
Article
in En
| MEDLINE
| ID: mdl-8315026
ABSTRACT
An enzyme immunoassay for detection of hepatitis B surface antigen based on the use of 3 monoclonal antibodies (mAbs) was developed an IgM as capture and 2 IgG1 for detection. The system biotin-streptavidin was compared with direct conjugation of mAbs to peroxidase and was preferred because of its higher signal to noise ratio. The possibility of simultaneous addition of human serum and biotin-mAb was discarded because of an evident prozone effect with some sera containing high HBsAg levels. The conjugation of biotin to IgG1 mAbs through a spacer arm (amidocaproyl) and the use of a highly sensitive substrate (tetramethylbenzIdine) improved the assay detection limit by about 10 times.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Hepatitis B Surface Antigens
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
J Immunoassay
Year:
1993
Document type:
Article
Affiliation country:
Venezuela