Isolation and characterization of deteriosomes from rat liver.
J Cell Biochem
; 51(4): 488-94, 1993 Apr.
Article
in En
| MEDLINE
| ID: mdl-8496249
ABSTRACT
Deteriosomes, a new class of microvesicles, have been isolated from rat liver tissue. These microvesicles are similar to those isolated previously from plant tissue [Yao et al., Proc Natl Acad Sci USA 882269-2273, 1991] in that they are nonsedimentable and enriched in membrane catabolites, particularly products of phospholipid degradation. Liver deteriosomes range in size from 0.05 microns to 0.11 microns in radius. They are also much more permeable than microsomal membrane vesicles indicating that the deteriosome bilayer is perturbed. The data are consistent with the proposal that deteriosomes are formed from membranes by microvesiculation and that they represent an intermediate stage of membrane deterioration. Furthermore, liver deteriosomes were found to contain phospholipase A2 activity. This suggests that they not only serve as a means of moving destabilizing macromolecular catabolites out of membranes into the cytosol but also possess enzymatic activity. The fact that the specific activity of phospholipase A2 is higher in deteriosomes than in deteriosome-free cytosol suggests that some of the enzymatic activity traditionally assumed to be cytosolic may in fact be associated with deteriosomes.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phospholipids
/
Organelles
/
Cell Fractionation
/
Liver
/
Membrane Lipids
Limits:
Animals
Language:
En
Journal:
J Cell Biochem
Year:
1993
Document type:
Article
Affiliation country:
Canada