Differential distribution of cAMP receptors cAR2 and cAR3 during Dictyostelium development.
Dev Biol
; 173(1): 353-6, 1996 Jan 10.
Article
in En
| MEDLINE
| ID: mdl-8575636
ABSTRACT
Signal transduction via a family of cAMP receptor subtypes (cARs) is critical for proper development in the cellular slime mold Dictyostelium. Genes encoding four related subtypes have been cloned and their expression, based on RNA accumulation, has been previously reported. Here we report the differential spatial and temporal distribution of cAR2 and cAR3 proteins, based on indirect double immunofluorescence. Cells were transformed with a carBlacZ construct, and an antibody against beta-galactosidase was used to visualize cAR2 expression. Simultaneously, a cAR3-specific antibody was used to identify cAR3-expressing cells. Results indicate that by the time of tip formation (12-14 hr) both receptors are expressed and distribute in a virtually nonoverlapping pattern, with cAR2 being expressed on anterior, prestalk cells and cAR3 present in the rest of the organism. Differential distribution of these two receptor subtypes may result in distinct cAMP signaling mechanisms in the two major regions of the organism.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, Cyclic AMP
/
Gene Expression Regulation, Developmental
/
Dictyostelium
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Dev Biol
Year:
1996
Document type:
Article
Affiliation country:
United States