The periplasmic TorT protein is required for trimethylamine N-oxide reductase gene induction in Escherichia coli.
J Bacteriol
; 178(4): 1219-23, 1996 Feb.
Article
in En
| MEDLINE
| ID: mdl-8576063
ABSTRACT
Expression of the Escherichia coli torCAD operon, which encodes the trimethylamine N-oxide reductase system, is regulated by the presence of trimethylamine N-oxide through the action of the TorR response regulator. We have identified an additional gene, torT, located just downstream from the torR gene, which is necessary for torCAD structural operon expression. Insertion within the torT gene dramatically reduced the expression of a torA'-'lacZ fusion, while presence of the gene in trans restored the wild-type phenotype. Overproduction of TorR in a torT strain resulted in partial constitutive expression of the torA'-'lacZ fusion, suggesting that TorR acts downstream from TorT. The torT gene codes for a 35.7-kDa periplasmic protein which presents some homology with the periplasmic ribose-binding protein of E. coli. We discuss the possible role of TorT as an inducer-binding protein involved in signal transduction of the tor regulatory pathway.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Gene Expression Regulation, Bacterial
/
Escherichia coli Proteins
/
Periplasmic Proteins
/
Periplasmic Binding Proteins
/
Escherichia coli
/
NADH, NADPH Oxidoreductases
Type of study:
Prognostic_studies
Language:
En
Journal:
J Bacteriol
Year:
1996
Document type:
Article
Affiliation country:
France