Purification of bacteriophage T4 DNA replication proteins.

Nossal, N G; Hinton, D M; Hobbs, L J; Spacciapoli, P

Nossal, N G; Hinton, D M; Hobbs, L J; Spacciapoli, P.

Affiliation

Nossal NG; Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20893-0830, USA.

Methods Enzymol ; 262: 560-84, 1995.

Article in En | MEDLINE | ID: mdl-8594379

Subject(s)

Bacteriophage T4/metabolism; DNA Replication; DNA-Binding Proteins/isolation & purification; DNA-Directed DNA Polymerase; Viral Proteins/isolation & purification; Animals; Bacteriophage T4/genetics; Chromatography/methods; Chromatography, Affinity/methods; Chromatography, DEAE-Cellulose/methods; Chromatography, Ion Exchange/methods; DNA/metabolism; DNA Helicases/isolation & purification; DNA Helicases/metabolism; DNA Ligases/isolation & purification; DNA Ligases/metabolism; DNA Primase; DNA, Single-Stranded/metabolism; DNA-Binding Proteins/metabolism; Durapatite; Electrophoresis, Polyacrylamide Gel/methods; Male; Mutagenesis; Plasmids; RNA Nucleotidyltransferases/isolation & purification; RNA Nucleotidyltransferases/metabolism; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Ribonuclease H/isolation & purification; Ribonuclease H/metabolism; Salmon; Spermatozoa; Viral Proteins/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: Viral Proteins / Bacteriophage T4 / DNA-Binding Proteins / DNA-Directed DNA Polymerase / DNA Replication Limits: Animals Language: En Journal: Methods Enzymol Year: 1995 Document type: Article Affiliation country: United States Country of publication: United States

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