Specific inhibitory effect of H1e histone somatic variant on in vitro DNA-methylation process.
Biochem Biophys Res Commun
; 220(1): 102-7, 1996 Mar 07.
Article
in En
| MEDLINE
| ID: mdl-8602826
ABSTRACT
H1e and H1c histone variants were purified from mouse L929 fibroblasts using a reverse phase HPLC, and their effect on in vitro DNA methylation was investigated, together with their ability to bind unmethylated or methylated CpG-rich 44bp oligonucleotides. In a "physiological" range of H1DNA ratios only H1e, at variance from H1c, was found to cause a marked inhibition of in vitro enzymic DNA methylation. It was also shown that both variants have a similar affinity in binding a methylated CpG-rich oligonucleotide, but that the binding to the same oligonucleotide in the unmethylated form occurs preferentially with H1e rather than with H1c. H1e is therefore likely to be directly involved in maintaining CpG-rich sequences in the unmethylated state.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Histones
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
1996
Document type:
Article
Affiliation country:
Italy