Short report: polymerase chain reaction detection of hepatitis E virus in north African fecal samples.
Am J Trop Med Hyg
; 54(2): 134-5, 1996 Feb.
Article
in En
| MEDLINE
| ID: mdl-8619435
ABSTRACT
Epidemics of enterically-transmitted non-A, non-B hepatitis were described in 1983-1984 involving French soldiers in Chad and in 1979-1980 in residents of Algeria. Hepatitis E virus (HEV) was subsequently implicated by serology. In this study, the presence of HEV in patient stool specimens from both outbreaks and from sporadic cases in residents of Chad (1994) was documented. This virus was detected in fecal suspensions by antibody capture of the virus and reverse transcriptase-polymerase chain reaction amplification of the viral RNA in the 3' end of open reading frame 2. Two of five epidemic cases from Chad (1983-1984) were positive, as well as one of five sporadic cases from Chad (1994), and two of three epidemic cases from Algeria (1979-1980). Of these 13 patients, 12 had detectable anti-HEV IgG in their serum. These results confirmed that HEV was the cause of hepatitis in at least five of these 13 patients.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymerase Chain Reaction
/
Hepatitis E virus
/
Feces
Type of study:
Diagnostic_studies
Limits:
Humans
Country/Region as subject:
Africa
Language:
En
Journal:
Am J Trop Med Hyg
Year:
1996
Document type:
Article
Affiliation country:
France