Ionic conductivity, transference numbers, composition and mobility of ions in cross-linked lysozyme crystals.
Biophys Chem
; 60(1-2): 1-16, 1996 May 13.
Article
in En
| MEDLINE
| ID: mdl-8645857
ABSTRACT
Micromethods for measurements of electric conductivity, transference numbers and concentrations of inorganic ions within immobilized protein crystals have been developed and applied to study tetragonal lysozyme crystals cross-linked with glutaraldehyde. Donnan equilibria and mobilities of ions in this crystal were calculated using the data of these methods and the data of crystal pH titration. Taken together these results characterize the lysozyme crystal as an ion exchanger whose electrical properties and ion composition differ greatly from those of the external solution. Although anions transfer most of the current in the crystals, anion mobility is considerably lower than that of cations. Mobility of all ions in the crystal is considerably lower than in solution (3.5-50 times for cations and 120-330 times for anions) and depends on steric restrictions and charges of both ions and lysozyme molecules. Similar features in behavior of crystalline and biological channels are discussed.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Muramidase
Language:
En
Journal:
Biophys Chem
Year:
1996
Document type:
Article
Affiliation country:
RUSSIA