Characterisation of carbon dioxide-inducible genes of the marine bacterium, Pseudomonas sp. S91.
FEMS Microbiol Lett
; 140(1): 37-42, 1996 Jun 15.
Article
in En
| MEDLINE
| ID: mdl-8666199
ABSTRACT
Characterisation of two genes in Pseudomonas sp. S91 that are responsive to carbon dioxide is reported. These were identified by random transposon mutagenesis leading to fusion of the Escherichia coli lacZ reporter gene to the genes of interest. Expression of the genes' promoters was quantified by measuring the reporter gene product, beta-galactosidase. beta-Galactosidase synthesis was induced when cells were exposed to 10% CO2 on solid media or during growth in aqueous phase when the culture density was greater than 1 at 610 nm, in either rich or minimal media. Induction of beta-galactosidase synthesis was not due to increased alkalinity, onset of stationary phase, build up of soluble metabolites in the culture supernatant, or cell density-dependent signalling. The CO2-inducible gene fusions were not induced by other environmental conditions that are known to stimulate global regulators of environmental gene expression. Benzoic acid (2 mM) induced beta-galactosidase synthesis in one of the mutants indicating the Co2 response may involve the intracellular CO2 partial pressure/bicarbonate ion concentration/pH equilibrium.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudomonas
/
Carbon Dioxide
/
Genes, Bacterial
Language:
En
Journal:
FEMS Microbiol Lett
Year:
1996
Document type:
Article
Affiliation country:
Australia