Reconstitution of GTP-gamma-S-dependent phospholipase D activity with ARF, RhoA, and a soluble 36-kDa protein.
FEBS Lett
; 387(2-3): 141-4, 1996 Jun 03.
Article
in En
| MEDLINE
| ID: mdl-8674536
ABSTRACT
For activation of kidney membrane phospholipase D (PLD), cytosol is absolutely needed in addition to GTP-gamma-S. The active component of cytosol consists of three protein factors ADP-ribosylation factor, RhoA, and a soluble 36-kDa protein. Any combination of these two factors synergistically activates PLD to some extent, but the presence of the three factors causes full activation. The 36-kDa protein is stable at 60 degrees C but inactivated at 80 degrees C for 10 min. Tissue distribution of the 36-kDa protein roughly coincides with that of PLD, suggesting physiological relevance of the protein in the regulation of PLD.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phospholipase D
/
Guanosine 5'-O-(3-Thiotriphosphate)
/
GTP-Binding Proteins
Limits:
Animals
Language:
En
Journal:
FEBS Lett
Year:
1996
Document type:
Article
Affiliation country:
Japan
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM