The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex.
Genes Dev
; 10(13): 1699-708, 1996 Jul 01.
Article
in En
| MEDLINE
| ID: mdl-8682299
ABSTRACT
The mechanism by which pre-mRNAs are initially recognized by the splicing machinery is not well understood. In the yeast system, commitment complexes are the earliest identified splicing complexes. They contain pre-mRNA, U1 snRNP, and the splicing factor Mud2p and probably correspond to the mammalian E complexes, which contain pre-mRNA, U1 snRNP, and the splicing factor U2AF. To identify other yeast commitment complex components, we have characterized mutant strains that are synthetic lethal with viable U1 snRNA mutations. We report here that MUD13 is a nonessential gene that encodes the yeast homolog of CBP20, the small subunit of the vertebrate nuclear cap-binding complex (CBC). Characterization of splicing in the delta-MUD13 strain and extract indicates that Mud13p is a yeast splicing factor and is the second identified non-snRNP commitment complex component. The observations also suggest that CBC interacts with other commitment complex components as well as with the substrate cap. Taken together with the accompanying results for a mammalian system, our data indicate that cap-binding proteins as well as the pre-mRNA cap contribute to early steps in spliceosome assembly.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphoproteins
/
Ribonucleoproteins
/
Saccharomyces cerevisiae
/
RNA Splicing
/
RNA-Binding Proteins
/
Saccharomyces cerevisiae Proteins
/
Nuclear Cap-Binding Protein Complex
Type of study:
Prognostic_studies
Language:
En
Journal:
Genes Dev
Journal subject:
BIOLOGIA MOLECULAR
Year:
1996
Document type:
Article
Affiliation country:
United States