Co-operative interactions of oligonucleosomal DNA with the H1e histone variant and its poly(ADP-ribosyl)ated isoform.
Biochem J
; 316 ( Pt 2): 475-80, 1996 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-8687390
ABSTRACT
H1 histone somatic variants from L929 mouse fibroblasts were purified by reverse-phase HPLC. We analysed the ability of each H1 histone variant to allow the H1-H1 interactions that are essential for the formation of the higher levels of chromatin structure, and we investigated the role played by the poly(ADP-ribosyl)ation process. Cross-linking analysis showed that H1e is the only somatic variant which, when bound to DNA, is able to produce H1-H1 polymers; the size of polymers was decreased when H1e was enriched in its poly(ADP-ribosyl)ated isoform. Measurement of the methyl-accepting ability in native nuclei compared with nuclei in which poly(ADP-ribosyl)ation was induced showed that the poly(ADP-ribosyl)ated H1 histone had not been removed from linker regions, in spite of its different interaction with DNA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Poly Adenosine Diphosphate Ribose
/
DNA
/
Histones
/
Nucleosomes
Limits:
Animals
Language:
En
Journal:
Biochem J
Year:
1996
Document type:
Article
Affiliation country:
Italy