S-phase induction by adenovirus E1A requires activation of cdc25a tyrosine phosphatase.
Oncogene
; 12(12): 2549-54, 1996 Jun 20.
Article
in En
| MEDLINE
| ID: mdl-8700513
ABSTRACT
Adenovirus E1A proteins can induce quiescent cells to enter S-phase and also affect the expression of cellular genes including various cell cycle regulators. Here we show that human cdc25A, a tyrosine phosphatase involved in regulation of the G1/S-phase transition of the cell cycle, is a target of the adenovirus E1A protein in virus-infected human fibroblasts. Expression of E1A in quiescent fibroblasts leads to a rapid increase in cdc25A phosphatase activity and also increases both cdc25A and cyclin E gene expression. Inhibition of cdc25A function by antibody injection prevents virus-induced entry into S-phase. These results indicate that induction of high levels of cdc25A and its potential positive regulator cyclin E mediates the ability of E1A to induce S-phase in the presence of antiproliferative signals.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
S Phase
/
Adenovirus E1A Proteins
/
Phosphoprotein Phosphatases
/
Cell Cycle Proteins
Limits:
Humans
Language:
En
Journal:
Oncogene
Journal subject:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Year:
1996
Document type:
Article
Affiliation country:
Germany