Simian immunodeficiency virus infection of CD8+ lymphocytes in vivo.
J Virol
; 70(8): 5646-50, 1996 Aug.
Article
in En
| MEDLINE
| ID: mdl-8764081
ABSTRACT
To determine the lymphoid target cells of simian immunodeficiency virus (SIV) in vivo, peripheral blood lymphocytes (PBL) and lymph node lymphocytes (LNL) were positively selected (>97% purity) for surface expression of CD4, CD8, or CD20 and then analyzed for SIV provirus using semiquantitative DNA amplification. We found provirus in CD4+ and CD8+ lymphocytes but none in CD20+ lymphocytes. During acute SIV infection (< or = 214 days postinoculation), the percentage of PBL and LNL CD4+ cells containing proviral DNA ranged from 0.2 to 20% and from 0.2 to 2%, respectively. Proviral burden in the CD8+ population of either PBL or LNL ranged from 0.01 to 0.2%. Virus isolation by cocultivation was positive for both CD4+ and CD8+ purified populations. No difference in proviral burden was observed between PBL and LNL subsets during acute SIV infection. Up to 19.4% of positively selected CD8+ cells also expressed CD4, and thus the provirus may reside within a dual-positive population. This dual-positive population may represent activated lymphocytes that are particularly susceptible to infection and may provide an opportunity for virus entry into the CD8+ CD4- lymphocytes in vivo.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Simian Acquired Immunodeficiency Syndrome
/
Simian Immunodeficiency Virus
/
CD8-Positive T-Lymphocytes
Limits:
Animals
Language:
En
Journal:
J Virol
Year:
1996
Document type:
Article
Affiliation country:
United States