Crystallization and preliminary crystallographic studies of 3-deoxy-D-manno-octulosonate-8-phosphate synthase from Escherichia coli.
Proteins
; 24(3): 407-8, 1996 Mar.
Article
in En
| MEDLINE
| ID: mdl-8778790
ABSTRACT
3-Deoxy-D-manno-octulosonate-8-phosphate (KDOP) synthase catalyzes the production of KDOP from phosphoenolpyruvate (PEP) and arabinose-5-phosphate (A5P). In gram-negative bacteria KDOP is subsequently dephosphorylated, cytidylylated, and linked to lipid A and is required for lipid A incorporation into the outer membrane (Raetz, Annu. Rev. Biochem. 59129-170, 1990). We have crystallized two forms of KDOP synthase belonging to space groups I23 or I2(1)3, one with a = b = c = 118.0 A and the other with a = b = c = 233 A.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aldehyde-Lyases
/
Escherichia coli
Language:
En
Journal:
Proteins
Journal subject:
BIOQUIMICA
Year:
1996
Document type:
Article
Affiliation country:
United States