Gonadal transformation in male Rana catesbeiana tadpoles intraperitoneally implanted with estradiol capsules.

ABSTRACT

Tadpoles of Rana catesbeiana at TK stages X-XII, about 9 months old, were laparotomized, and the males were implanted intraperitoneally with silastic tubes containing estradiol (E2) for various periods. Male tadpoles implanted with empty tubes served as the controls. Histology, secretions of E2, and testosterone (T) in the gonads were investigated. A rough estimate of estradiol released from silastic tubes suggested that about 90 micrograms per tadpole in 6 months. Histological observation revealed various degrees of transformation from testes toward ovaries in E2-treated testes. Ten in thirteen (77%), the testes were transformed into ovaries 6 months after the treatment. The testes of the controls, however, displayed normal histology. Radioimmunoassay showed that E2 level was increased while T level was decreased in E2-treated testes. These results indicate that a low dose of exogenous E2 may induce transformation of the testes into ovaries.