Analysis of FeLV-FAIDS provirus burden and productive infection in lymphocyte subsets in vivo.
Virology
; 223(1): 1-9, 1996 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-8806534
ABSTRACT
To help elucidate the immunopathogenesis of feline leukemia virus (FeLV)-induced immunodeficiency we studied the tropism of viruses derived from the FeLV-FAIDS isolate for lymphocyte subpopulations in cats. FeLV-FAIDS is composed of a replication-competent virus typical of subgroup A FeLV (prototype, clone 61E) and a family of replication-defective but immunopathogenic variant viruses (prototype, clone 61C). We sorted CD4+, CD8+, and IgG+ lymphocytes to > or = 97% purity and analyzed viral load in each cell population via genome-specific semiquantitative PCR. Both the 61E and 61C viruses were tropic for CD4+ and CD8+ T cells as well as IgG+ B lymphocytes in blood and lymph node. High provirus burden were established for both virus genomes-ranging from 0.3 to > 2 copies/cell. To identify the fraction of circulating cells which expressed viral antigen in vivo, we developed a flow cytometric method to simultaneously label blood leukocytes for surface immunophenotype and intracytoplasmic FeLV CA (p27 Gag). These experiments established that 20 to 60% of CD4+, CD8+, and IgG+ lymphocytes and > 85% of monocytes and granulocytes expressed FeLV p27 intracellularly. Thus the in vivo target cells for FeLV-FAIDS infection are manifold and include CD4+ and CD8+ T cells, B cells, and myeloid cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Feline Acquired Immunodeficiency Syndrome
/
B-Lymphocyte Subsets
/
T-Lymphocyte Subsets
/
Leukemia Virus, Feline
Limits:
Animals
Language:
En
Journal:
Virology
Year:
1996
Document type:
Article
Affiliation country:
United States