Liver specific kynurenine(alanine):glyoxylate aminotransferase was expressed in kidney cell line.

ABSTRACT

Kynurenine (Alanine); glyoxylate aminotransferase (AGT) expression plasmid in the COS-7 was constructed. pGV-C was used as a expression vector which contains SV-40 promoter and enhancer. pGV-C and human AGT clone, H1-2, were digested by Hind III/Sma I separately. The AGT fragment was inserted into the digested pGV-C large fragment, The constructed plasmid was named as pGV-AGT. The constructed plasmid was transfected to COS-7 cultured cell by electroporation. The best electroporation condition was checked.