Design of hammerhead ribozymes to distinguish single base changes in substrate RNA.
Antisense Nucleic Acid Drug Dev
; 6(3): 165-8, 1996.
Article
in En
| MEDLINE
| ID: mdl-8915500
ABSTRACT
Hammerhead ribozymes are attractive tools in antisense gene inactivation because of their catalytic cleavage of target molecules. High sequence discrimination should be possible, since the cleavage efficiencies were already significantly reduced, if single base changes in substrate RNA introduce mismatches next to the cleavage site. This was observed at the first innermost base pair in helix I and the two innermost base pairs in helix III. In addition to its position, the nature of the mismatch pair was important.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Drug Design
/
RNA, Catalytic
/
Sequence Analysis, RNA
Language:
En
Journal:
Antisense Nucleic Acid Drug Dev
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
/
FARMACOLOGIA
Year:
1996
Document type:
Article
Affiliation country:
Germany