Simultaneous determination of azathioprine and 6-mercaptopurine by high-performance liquid chromatography.
J Chromatogr B Biomed Appl
; 679(1-2): 147-54, 1996 Apr 26.
Article
in En
| MEDLINE
| ID: mdl-8998554
ABSTRACT
A specific, sensitive, single-step solid-phase extraction and reversed-phase high-performance liquid chromatographic method for the simultaneous determination of plasma 6-mercaptopurine and azathioprine concentrations is reported. Following solid-phase extraction, analytes are separated on a C18 column with mobile phase consisting of 0.8% acetonitrile in 1 mM triethylamine, pH 3.2, run on a gradient system. Quantitation limits were 5 ng/ml and 2 ng/ml for azathioprine and 6-mercaptopurine, respectively. Peak heights correlated linearly to known extracted standards for 6-mercaptopurine and azathioprine (r = 0.999) over a range of 2-200 ng/ml. No chromatographic interferences were detected.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Azathioprine
/
Chromatography, High Pressure Liquid
/
Antirheumatic Agents
/
Immunosuppressive Agents
/
Mercaptopurine
Limits:
Adult
/
Female
/
Humans
Language:
En
Journal:
J Chromatogr B Biomed Appl
Year:
1996
Document type:
Article
Affiliation country:
United States