Clonality analysis of hematopoietic cell lineages in acute myeloid leukemia and translocation (8;21): only myeloid cells are part of the malignant clone.
Leukemia
; 11(2): 202-5, 1997 Feb.
Article
in En
| MEDLINE
| ID: mdl-9009081
ABSTRACT
Bone marrow from six patients with acute myeloid leukemia (AML) and t(8;21) (q22;q22) or a variant t(8;13;21) was studied by simultaneous analysis of cell morphology and karyotype. Combination of May-Grünwald-Giemsa (MGG) and fluorescence in situ hybridization (FISH) using probes specific for the breakpoint regions of chromosome 8 and 21 allowed us to establish the extent of cell-lineage involvement of the translocation. The translocation was found in all myeloid blasts and in high percentages of the more mature neutrophilic cells. In one patient we could demonstrate the translocation in the eosinophils as well. Erythroblasts and lymphocytes did not show the t(8;21) abnormality. These results indicate that the t(8;21) in AML is restricted to the myeloid (granulocytic) lineage.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Translocation, Genetic
/
Neoplastic Stem Cells
/
Chromosomes, Human, Pair 8
/
Chromosomes, Human, Pair 21
/
Hematopoietic Stem Cells
/
Leukemia, Myeloid, Acute
/
Clone Cells
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Leukemia
Journal subject:
HEMATOLOGIA
/
NEOPLASIAS
Year:
1997
Document type:
Article
Affiliation country:
Netherlands