Guide RNA-independent and guide RNA-dependent uridine insertion into cytochrome b mRNA in a mitochondrial lysate from Leishmania tarentolae. Role of RNA secondary structure.
J Biol Chem
; 272(7): 4212-8, 1997 Feb 14.
Article
in En
| MEDLINE
| ID: mdl-9020135
A primer extension assay was used for the detection of uridine insertions occurring in vitro in synthetic pre-edited cytochrome b mRNA during incubation with a Leishmania tarentolae mitochondrial extract. Two different activities were detected that inserted uridines within the first two editing sites: one that is dependent on the secondary structure of the mRNA but is independent of both exogenous and endogenous guide RNA, and a second that does not put the same structural constraints on the mRNA, but is dependent on the presence of a cognate guide RNA.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Uridine
/
RNA, Messenger
/
RNA, Guide, Kinetoplastida
/
Cytochrome b Group
/
Leishmania
/
Mitochondria
Limits:
Animals
Language:
En
Journal:
J Biol Chem
Year:
1997
Document type:
Article
Affiliation country:
United States
Country of publication:
United States