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Guide RNA-independent and guide RNA-dependent uridine insertion into cytochrome b mRNA in a mitochondrial lysate from Leishmania tarentolae. Role of RNA secondary structure.
Connell, G J; Byrne, E M; Simpson, L.
Affiliation
  • Connell GJ; Howard Hughes Medical Institute, University of California, Los Angeles, California 90095-1662, USA.
J Biol Chem ; 272(7): 4212-8, 1997 Feb 14.
Article in En | MEDLINE | ID: mdl-9020135
A primer extension assay was used for the detection of uridine insertions occurring in vitro in synthetic pre-edited cytochrome b mRNA during incubation with a Leishmania tarentolae mitochondrial extract. Two different activities were detected that inserted uridines within the first two editing sites: one that is dependent on the secondary structure of the mRNA but is independent of both exogenous and endogenous guide RNA, and a second that does not put the same structural constraints on the mRNA, but is dependent on the presence of a cognate guide RNA.
Subject(s)
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Collection: 01-internacional Database: MEDLINE Main subject: Uridine / RNA, Messenger / RNA, Guide, Kinetoplastida / Cytochrome b Group / Leishmania / Mitochondria Limits: Animals Language: En Journal: J Biol Chem Year: 1997 Document type: Article Affiliation country: United States Country of publication: United States
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Collection: 01-internacional Database: MEDLINE Main subject: Uridine / RNA, Messenger / RNA, Guide, Kinetoplastida / Cytochrome b Group / Leishmania / Mitochondria Limits: Animals Language: En Journal: J Biol Chem Year: 1997 Document type: Article Affiliation country: United States Country of publication: United States