A novel, rapid, and highly sensitive mass assay for phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) and its application to measure insulin-stimulated PtdIns(3,4,5)P3 production in rat skeletal muscle in vivo.

van der Kaay, J; Batty, I H; Cross, D A; Watt, P W; Downes, C P

van der Kaay, J; Batty, I H; Cross, D A; Watt, P W; Downes, C P.

Affiliation

van der Kaay J; Department of Biochemistry, Medical Sciences Institute, University of Dundee, DD1 4HN Dundee, United Kingdom. jvdkaay@bad.dundee.ac.uk

J Biol Chem ; 272(9): 5477-81, 1997 Feb 28.

Article in En | MEDLINE | ID: mdl-9038150

Subject(s)

Insulin/pharmacology; Muscle, Skeletal/drug effects; Phosphatidylinositol Phosphates/analysis; Phosphotransferases (Alcohol Group Acceptor)/metabolism; Animals; Brain/enzymology; Hydrogen-Ion Concentration; Kinetics; Muscle, Skeletal/metabolism; Phosphatidylinositol 3-Kinases; Phosphatidylinositol Phosphates/metabolism; Rats; Sensitivity and Specificity; Tumor Cells, Cultured

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Collection: 01-internacional Database: MEDLINE Main subject: Phosphotransferases (Alcohol Group Acceptor) / Phosphatidylinositol Phosphates / Muscle, Skeletal / Insulin Type of study: Diagnostic_studies Limits: Animals Language: En Journal: J Biol Chem Year: 1997 Document type: Article Affiliation country: United kingdom

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