Evaluation of spontaneous and chemical-induced lacI mutations in germ cells from lambda/lacI transgenic mice.
Mutat Res
; 388(2-3): 137-43, 1997 Feb 14.
Article
in En
| MEDLINE
| ID: mdl-9057874
ABSTRACT
The spontaneous mutant frequency in germ cells isolated from seminiferous tubules of two lambda/lacI transgenic mouse strains, C57BL/6 and B6C3F1 was evaluated. At least 500 000 phage were screened for mutation at lacI for each animal using standardized assay procedures. The germ cell spontaneous lacI mutant frequency was 17.8 +/- 8.1 x 10(-6) in C57BL/6 mice and 17.0 +/- 10.0 x 10(-6) in B6C3F1 mice. The induction of germ cell mutations by three well characterized alkylating agents were also evaluated in C57BL/6 mice on day 3 after a single dose administration. The lacI mutant frequencies were significantly elevated in transgenic mice dosed with ENU at 150 mg/kg (2-fold increase above control) and iPMS at 200 mg/kg (3-fold increase above control) but not in those receiving MMS at 40 mg/kg. These findings suggest that single dose studies using the lambda/lacI transgenic system may be capable of detecting germ mutations induced by chemicals characterized either by point mutations or small, intragenic deletions but not those characterized by a predominance of multi-locus deletions.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Repressor Proteins
/
Spermatozoa
/
Bacterial Proteins
/
Germ-Line Mutation
/
Escherichia coli Proteins
/
Mutagenicity Tests
Limits:
Animals
Language:
En
Journal:
Mutat Res
Year:
1997
Document type:
Article
Affiliation country:
United States
Publication country:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS