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Developmentally ordered V-J recombination in mouse T cell receptor gamma locus is not perturbed by targeted deletion of the Vgamma4 gene.
Sunaga, S; Maki, K; Komagata, Y; Miyazaki, J; Ikuta, K.
Affiliation
  • Sunaga S; Department of Disease-Related Gene Regulation Research (Sandoz), Faculty of Medicine, University of Tokyo, Japan.
J Immunol ; 158(9): 4223-8, 1997 May 01.
Article in En | MEDLINE | ID: mdl-9126983
Mouse TCR gamma genes in the gamma1 cluster are arranged in the order of Vgamma5, Vgamma2, Vgamma4, Vgamma3, Jgamma1, and Cgamma1 on the chromosome. During thymic ontogeny, each Vgamma gene recombines with the Jgamma1 gene in the order of proximity to Jgamma1. To explore the mechanism of the ordered recombination, we generated Vgamma4-deficient mice by gene targeting and the Cre/loxP system, by deleting the 4.8-kb DNA region between 3' of the Vgamma2 and 3' of the Vgamma4. In semiquantitative PCR analysis, Vgamma2-Jgamma1 recombination was detected frequently in adult thymus, while Vgamma3-Jgamma1 recombination preferentially occurred in fetal thymus of the mutant mice. There was no difference in the frequency of V-J recombinations between control and mutant mice. Southern blot analysis also revealed that recombination of the Vgamma2 gene occurred as frequently as in control mice. In addition, there was no difference in the levels of germline transcripts of Vgamma2 and Vgamma3 genes between control and mutant mice. Therefore, regulation of the Vgamma-Jgamma recombination was not affected by deletion of the Vgamma4 gene. These results suggest that the ordered recombination is controlled by regulatory elements near each Vgamma gene.
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Collection: 01-internacional Database: MEDLINE Main subject: T-Lymphocytes / Receptors, Antigen, T-Cell, gamma-delta / Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor Limits: Animals Language: En Journal: J Immunol Year: 1997 Document type: Article Affiliation country: Japan Country of publication: United States
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Collection: 01-internacional Database: MEDLINE Main subject: T-Lymphocytes / Receptors, Antigen, T-Cell, gamma-delta / Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor Limits: Animals Language: En Journal: J Immunol Year: 1997 Document type: Article Affiliation country: Japan Country of publication: United States