On the stability of a tumour cell surface protease after exposure to 6N HCl.
Anticancer Res
; 17(2A): 891-3, 1997.
Article
in En
| MEDLINE
| ID: mdl-9137423
ABSTRACT
Well defined acinar tumour cells in frozen sections were exposed to 6NHCl for 5 h at room temperature. A technique was designed to monitor the activity of the enzyme, guanidinobenzoatase (GB), on these tumour cells; this involved cross-linking the enzyme to the cell surface and challenging the active centre with known fluorescent probes which only bind to the functional enzyme. It was demonstrated that the enzymic activity can be regained by appropriate folding of the protein.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endopeptidases
/
Carboxylic Ester Hydrolases
/
Neoplasms
Limits:
Humans
Language:
En
Journal:
Anticancer Res
Year:
1997
Document type:
Article
Affiliation country:
United kingdom
Publication country:
GR
/
GRECIA
/
GREECE
/
GRÉCIA