An ELISA for detection of antibodies to the E2 protein of GB virus C.
J Infect Dis
; 175(2): 458-61, 1997 Feb.
Article
in En
| MEDLINE
| ID: mdl-9203673
ABSTRACT
An ELISA was developed for detection of antibodies to GB virus C (GBV-C) using a recombinant E2 protein expressed in CHO cells. Seroconversion to anti-E2 positivity was noted among several persons infected with GBV-C RNA-positive blood through transfusion. Of 6 blood recipients infected by GBV-C RNA-positive donors, 4 (67%) became anti-E2 positive and cleared their viremia. Thus, anti-E2 seroconversion is associated with viral clearance. The prevalence of antibodies to E2 was relatively low (3.0%-8.1%) in volunteer blood donors but was higher in several other groups, including plasmapheresis donors (34.0%), intravenous drug users (85.2%), and West African subjects (13.3%), all of whom tested negative by GBV-C reverse-transcription polymerase chain reaction (RT-PCR). These data demonstrate that testing for anti-E2 should greatly extend the ability of RT-PCR to define the epidemiology and clinical significance of GBV-C.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Viral Envelope Proteins
/
Flaviviridae
/
Hepatitis, Viral, Human
/
Antibodies, Viral
Type of study:
Diagnostic_studies
/
Prevalence_studies
/
Risk_factors_studies
Limits:
Animals
/
Humans
Country/Region as subject:
Africa
Language:
En
Journal:
J Infect Dis
Year:
1997
Document type:
Article
Affiliation country:
United States