Comparison of a monoclonal antibody-based IgM capture ELISA with a neutralization assay for assessing response to trivalent oral poliovirus vaccine.
J Infect Dis
; 175 Suppl 1: S264-7, 1997 Feb.
Article
in En
| MEDLINE
| ID: mdl-9203727
ABSTRACT
Monoclone-based IgM capture ELISAs were developed for each of the three poliovirus serotypes and compared with a neutralization assay for detecting response to trivalent oral poliovirus vaccine among 224 infants. The IgM-based response rates were significantly higher than the neutralizing antibody-based rates 95% versus 83% to poliovirus type 1, 99% versus 94% to poliovirus type 2, and 89% versus 59% to poliovirus type 3. IgM responses to the first vaccine dose were significantly associated between serotypes, suggesting that some of the discordance may reflect a heterotypic IgM response. When the response rates in 4 vaccine formulation groups were compared, group differences using the two assays were similar for poliovirus types 1 and 2 but not for type 3. Therefore, IgM results using these assays may not be adequate substitutes for neutralizing antibody results when determining vaccine response.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Poliomyelitis
/
Enzyme-Linked Immunosorbent Assay
/
Neutralization Tests
/
Poliovirus Vaccine, Oral
/
Poliovirus
Limits:
Animals
/
Humans
/
Infant
Language:
En
Journal:
J Infect Dis
Year:
1997
Document type:
Article
Affiliation country:
United States