beta-Galactosidase reporter system in mycobacteria and its application in rapid antimycobacterial drug screening.
Biochem Biophys Res Commun
; 235(3): 602-5, 1997 Jun 27.
Article
in En
| MEDLINE
| ID: mdl-9207204
ABSTRACT
Pathogenic mycobacteria are generally slow growing organisms and it takes several weeks to evaluate inhibitors of growth. Therefore, for rapid screening of the inhibitors of mycobacterial growth, a beta-galactosidase reporter system has been described which utilises a recombinant Mycobacterium smegmatis mc(2)155 expressing E. coli lacZ gene as the test organism. The assay is based on production of beta-galactosidase in presence of drugs during growth. A correlation between beta-galactosidase production and colony forming ability of mycobacteria was obtained. beta-galactosidase production was inhibited within 6 h by front line standard antimycobacterial drugs like streptomycin, rifampicin, isoniazid, ethambutol, pyrazinamide and ofloxacin at their reported MICs. The assay was performed on mycobacterial cells permeabilized with chloroform and sodium dodecyl sulfate.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microbial Sensitivity Tests
/
Beta-Galactosidase
/
Anti-Bacterial Agents
/
Mycobacterium
/
Antitubercular Agents
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
1997
Document type:
Article
Affiliation country:
India