Incidence of aneuploid spermatozoa among infertile men studied by multicolor fluorescence in situ hybridization.
Am J Med Genet
; 71(1): 115-21, 1997 Jul 11.
Article
in En
| MEDLINE
| ID: mdl-9215780
ABSTRACT
We studied by fluorescence in situ hybridization the frequency of aneuploidy in spermatozoa of 12 infertile men 8 with normal or nearly normal semen analysis values and 4 with oligo-astheno-teratozoospermia. The control group consisted of 18 normal healthy fertile men. Probes for chromosome 1 and 7 were used and 10,000 spermatozoa per individual were scored. The hybridization efficiency was good (higher than 98%). In the group with nearly normal semen analysis values the frequencies of spermatozoa disomic for chromosome 1 or chromosome 7 were 0.08% and 0.07%, respectively, and not elevated compared to controls (0.10% and 0.06%, respectively). The frequency of diploid spermatozoa was 0.17%, not significantly different from the control group (0.15%) either. In the group of oligoastheno-teratozoospermic men both the frequencies of disomic cells for chromosome 1 (0.22%) and for chromosome 7 (0.13%) and of diploid spermatozoa (0.56%) were significantly higher compared to controls, although this was mainly due to one patient with high frequencies of hyperploid sperm. The results indicate that infertility may be a risk factor for chromosomal aneuploidy in spermatozoa.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatozoa
/
Infertility, Male
/
Aneuploidy
Type of study:
Incidence_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Adult
/
Humans
/
Male
Language:
En
Journal:
Am J Med Genet
Year:
1997
Document type:
Article
Affiliation country:
Finland