Multianalyte biosensors on optical imaging bundles.

ABSTRACT

We present an optical biosensor design that expands the utility of enzyme biosensors. These biosensors are fabricated by site-selective photodeposition of analyte-sensitive polymer matrices on optical imaging fibres. These dual-analyte arrays allow for the simultaneous, independent measurement of the analyte of interest and the transducing analyte. The first integrated optical-biosensors using this design have been prepared that allow both the dependent and independent analytes to be measured simultaneously, for example penicillin and pH (Healey & Walt, 1995) or glucose and O2 (Li & Walt, 1995). Independent measurement of the transducing analyte allows penicillin or glucose to be quantitated in the presence of a concurrent pH or O2 change, respectively. Penicillin can be measured in the range 0.25-10.0 mM in the pH range 6.2-7.5. Glucose can be measured in the range 0.6-20.0 mM in the O2 range 20-100%. The utility of the sensor design was demonstrated by using the penicillin-dual-analyte biosensor to quantitate penicillin produced during a Penicillium chrysogenum fermentation.