Enhancement of the immune response to non-replicating herpes simplex virus type-1 preparations by mucosal administration in the presence of cholera toxin.
Vaccine
; 15(10): 1065-9, 1997 Jul.
Article
in En
| MEDLINE
| ID: mdl-9269048
ABSTRACT
Different immunization regimes were compared in order to enhance the immune response following mucosal administration of non-replicating HSV-1 preparations to mice. The serum anti-HSV Ig response following intragastric administration of heat or UV inactivated HSV-1 strain SC16 was compared with that elicited by an attenuated derivative of SC16 (TKDM21). The highest response followed immunization with TKDM21 and this was markedly enhanced by repeated intragastric administration, reaching ca 35% of that elicited following a cutaneous infection with live virus. Repeated doses of killed virus produced only a minimal increase in the response even when given intranasally (i.n.). However, cholera toxin and its B-subunit with UV-inactivated virus or a mixture of purified viral glycoproteins enhanced the anti-HSV response after i.n. immunization and produced antibody levels equivalent to those following intragastric delivery of live TKDM21.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Vaccines
/
Cholera Toxin
/
Herpesvirus 1, Human
Limits:
Animals
Language:
En
Journal:
Vaccine
Year:
1997
Document type:
Article
Affiliation country:
United kingdom
Publication country:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS