Rapid colorectal adenoma formation initiated by conditional targeting of the Apc gene.
Science
; 278(5335): 120-3, 1997 Oct 03.
Article
in En
| MEDLINE
| ID: mdl-9311916
ABSTRACT
Familial adenomatous polyposis coli (FAP) is a disease characterized by the development of multiple colorectal adenomas, and affected individuals carry germline mutations in the APC gene. With the use of a conditional gene targeting system, a mouse model of FAP was created that circumvents the embryonic lethality of Apc deficiency and directs Apc inactivation specifically to the colorectal epithelium. loxP sites were inserted into the introns around Apc exon 14, and the resultant mutant allele (Apc580S) was introduced into the mouse germline. Mice homozygous for Apc580S were normal; however, upon infection of the colorectal region with an adenovirus encoding the Cre recombinase, the mice developed adenomas within 4 weeks. The adenomas showed deletion of Apc exon 14, indicating that the loss of Apc function was caused by Cre-loxP-mediated recombination.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Proteins
/
Genes, APC
/
Adenomatous Polyposis Coli
/
Gene Targeting
Limits:
Animals
Language:
En
Journal:
Science
Year:
1997
Document type:
Article
Affiliation country:
Japan
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA