Direct ligation of human CD4 polymerase chain reaction fragment into vectors at specific restriction sites with positional heterostagger cloning.

Felfoldi, F; Kupihar, Z; Bottka, S; Puskas, L G

Felfoldi, F; Kupihar, Z; Bottka, S; Puskas, L G.

Affiliation

Felfoldi F; Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.

Anal Biochem ; 253(2): 275-7, 1997 Nov 15.

Article in En | MEDLINE | ID: mdl-9367518

Subject(s)

CD4 Antigens/genetics; Cloning, Molecular/methods; DNA Restriction Enzymes/metabolism; Genetic Vectors/metabolism; Polymerase Chain Reaction/methods; DNA-Directed DNA Polymerase/metabolism; Humans; Models, Molecular; Taq Polymerase/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: DNA Restriction Enzymes / CD4 Antigens / Polymerase Chain Reaction / Cloning, Molecular / Genetic Vectors Limits: Humans Language: En Journal: Anal Biochem Year: 1997 Document type: Article Affiliation country: Hungary

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