A RIPE3b1-like factor binds to a novel site in the human insulin promoter in a redox-dependent manner.
FEBS Lett
; 418(1-2): 68-72, 1997 Nov 24.
Article
in En
| MEDLINE
| ID: mdl-9414097
ABSTRACT
In the human insulin gene, a regulatory sequence upstream of the transcription start site at -229 to -258 (the E2 element) binds a ubiquitous factor USF. The present study led to the identification of a second factor, D0, that binds to an adjacent upstream site, the C2 element, that has previously not been described. The results demonstrate that D0 exhibits similar properties to RIPE3b1, a factor shown to be an important determinant of insulin gene beta-cell-specific expression. Binding of D0 to the C2 element was abolished by the oxidising agent diamide, and the alkylating agent N-ethylmaleimide. The results indicate that expression of the insulin gene may be regulated by a redox-dependent pathway involving RIPE3b1 or a RIPE3b1-like factor.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Regulatory Sequences, Nucleic Acid
/
Promoter Regions, Genetic
/
DNA-Binding Proteins
/
Insulin
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
FEBS Lett
Year:
1997
Document type:
Article
Affiliation country:
United kingdom
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM