Integrated amplification and detection of the C677T point mutation in the methylenetetrahydrofolate reductase gene by fluorescence resonance energy transfer and probe melting curves.

Bernard, P S; Lay, M J; Wittwer, C T

Bernard, P S; Lay, M J; Wittwer, C T.

Affiliation

Bernard PS; Department of Pathology, University of Utah Medical School, Salt Lake City 84132, USA.

Anal Biochem ; 255(1): 101-7, 1998 Jan 01.

Article in En | MEDLINE | ID: mdl-9448847

Subject(s)

Genes; Oxidoreductases Acting on CH-NH Group Donors/genetics; Alleles; DNA/chemistry; DNA/genetics; Energy Transfer; Fluorescent Dyes; Gene Frequency; Genotype; Heterozygote; Homozygote; Hot Temperature; Methylenetetrahydrofolate Reductase (NADPH2); Nucleic Acid Denaturation; Nucleic Acid Heteroduplexes/chemistry; Point Mutation/genetics; Point Mutation/physiology; Polymerase Chain Reaction; Spectrometry, Fluorescence

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Collection: 01-internacional Database: MEDLINE Main subject: Oxidoreductases Acting on CH-NH Group Donors / Genes Type of study: Diagnostic_studies Language: En Journal: Anal Biochem Year: 1998 Document type: Article Affiliation country: United States

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