Effects of immunotherapy on experimental immunodeficiency-related lymphoproliferative disease.
Transplantation
; 65(2): 264-8, 1998 Jan 27.
Article
in En
| MEDLINE
| ID: mdl-9458027
ABSTRACT
BACKGROUND:
Human lymphokine-activated cells (LAK cells) and interferon alpha (IFN-alpha) have been used clinically in the therapy of posttransplant lymphoproliferative disease (PTLD). However, the efficacy of such therapy has not been extensively tested under controlled experimental conditions.METHODS:
A B-cell line, derived from PTLD tissue and clonally related to the parent lesion, was tested for its response to IFN-alpha in vitro. The effects of LAK cells and IFN-alpha therapy were examined in a severe combined immunodeficiency disease (SCID) mouse model in vivo.RESULTS:
The PTLD cell line studied showed a 30% decrease in the rate of growth upon incubation with 500 U/ml of IFN-alpha. This in vitro response was also reproduced in vivo, in tumor therapy studies conducted in SCID mice. The magnitude of this inhibitory effect in vivo was a function of tumor burden and dose of IFN-alpha. In parallel experiments, LAK cells reduced the tumorigenicity of a lymphoblastoid cell line derived from the peripheral blood of a patient with PTLD, and prolonged the survival of SCID-beige mice with established lymphoproliferative disease. In contrast with two prior studies, in which the use of autologous cytotoxic T cells was found to be necessary, we found the administration of third-party non-HLA-matched LAK cells also to be effective in reducing tumor burden.CONCLUSIONS:
These observations demonstrate the efficacy of immunotherapy for lymphoproliferative disease under controlled experimental conditions, and validate currently ongoing efforts exploring the utility of such therapy in the clinical setting.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Killer Cells, Lymphokine-Activated
/
Immunotherapy, Adoptive
/
Interferon-alpha
/
Immunocompromised Host
/
Lymphoproliferative Disorders
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Transplantation
Year:
1998
Document type:
Article
Affiliation country:
United States