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Complete deficiency of glycophorin A in red blood cells from mice with targeted inactivation of the band 3 (AE1) gene.
Hassoun, H; Hanada, T; Lutchman, M; Sahr, K E; Palek, J; Hanspal, M; Chishti, A H.
Affiliation
  • Hassoun H; Laboratory of Tumor Cell Biology, Department of Biomedical Research, St. Elizabeth's Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA.
Blood ; 91(6): 2146-51, 1998 Mar 15.
Article in En | MEDLINE | ID: mdl-9490702
ABSTRACT
Glycophorin A is the major transmembrane sialoglycoprotein of red blood cells. It has been shown to contribute to the expression of the MN and Wright blood group antigens, to act as a receptor for the malaria parasite Plasmodium falciparum and Sendai virus, and along with the anion transporter, band 3, may contribute to the mechanical properties of the red blood cell membrane. Several lines of evidence suggest a close interaction between glycophorin A and band 3 during their biosynthesis. Recently, we have generated mice where the band 3 expression was completely eliminated by selective inactivation of the AE1 anion exchanger gene, thus allowing us to study the effect of band 3 on the expression of red blood cell membrane proteins. In this report, we show that the band 3 -/- red blood cells contain protein 4.1, adducin, dematin, p55, and glycophorin C. In contrast, the band 3 -/- red blood cells are completely devoid of glycophorin A (GPA), as assessed by Western blot and immunocytochemistry techniques, whereas the polymerase chain reaction (PCR) confirmed the presence of GPA mRNA. Pulse-label and pulse-chase experiments show that GPA is not incorporated in the membrane and is rapidly degraded in the cytoplasm. Based on these findings and other published evidence, we propose that band 3 plays a chaperone-like role, which is necessary for the recruitment of GPA to the red blood cell plasma membrane.
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Collection: 01-internacional Database: MEDLINE Main subject: Anion Exchange Protein 1, Erythrocyte / Glycophorins / Erythrocyte Membrane Limits: Animals Language: En Journal: Blood Year: 1998 Document type: Article Affiliation country: United States
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Collection: 01-internacional Database: MEDLINE Main subject: Anion Exchange Protein 1, Erythrocyte / Glycophorins / Erythrocyte Membrane Limits: Animals Language: En Journal: Blood Year: 1998 Document type: Article Affiliation country: United States