Suppression subtractive hybridization (SSH) identifies prolactin stimulation of p38 MAP kinase gene expression in Nb2 T lymphoma cells: molecular cloning of rat p38 MAP kinase.
J Mol Endocrinol
; 20(1): 151-6, 1998 Feb.
Article
in En
| MEDLINE
| ID: mdl-9513091
Suppression Subtractive Hybridization (SSH) has been used to compare rat Nb2 cells treated with prolactin for 1 hour with untreated cells. This new method for identifying differentially expressed genes showed that the mRNAs for at least three genes were elevated by such treatment, including a p38 mitogen activated protein (MAP) kinase. The p38 MAP kinase was cloned and the full length cDNA sequence was determined.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prolactin
/
Gene Expression Regulation, Enzymologic
/
Lymphoma, T-Cell
/
Calcium-Calmodulin-Dependent Protein Kinases
/
Mitogen-Activated Protein Kinases
Limits:
Animals
Language:
En
Journal:
J Mol Endocrinol
Journal subject:
BIOLOGIA MOLECULAR
/
ENDOCRINOLOGIA
Year:
1998
Document type:
Article
Affiliation country:
United States
Country of publication:
United kingdom