Selective quasispecies transmission after systemic or mucosal exposure of macaques to simian immunodeficiency virus.
Virology
; 243(1): 12-20, 1998 Mar 30.
Article
in En
| MEDLINE
| ID: mdl-9527911
ABSTRACT
Sexual transmission is the major cause of the AIDS epidemic. For the development of new antiviral and vaccine strategies, we therefore need to understand the mechanisms by which lentiviruses cross the mucosal barrier and the subsequent pathogenic consequences. For this purpose, experimental approaches are greatly facilitated by the development of relevant animal models. In this study, macaques were inoculated intravenously, intrarectally, or intravaginally with a pathogenic cell-free isolate of SIVmac251. Patterns of virological and immunological events significantly differed between vaginally (transient viremia, late seroconversion) and intravenously or intrarectally inoculated monkeys (persistent viremia and early seroconversion). Two weeks after infection, analysis of the env gene nucleotide sequences of proviruses recovered from PBMCs demonstrated that most of the differences were observed in the V1 loop. Three viral variants were specifically associated with vaginal transmission, whereas no such selection was evidenced after intravenous or intrarectal transmissions. These results are in favor of specific mechanisms associated with vaginal transmission, implicating viral envelope structure.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vagina
/
Membrane Glycoproteins
/
HIV Envelope Protein gp120
/
Viral Envelope Proteins
/
Simian Acquired Immunodeficiency Syndrome
/
Simian Immunodeficiency Virus
/
Intestinal Mucosa
Limits:
Animals
Language:
En
Journal:
Virology
Year:
1998
Document type:
Article
Affiliation country:
France