Site-directed mutagenesis of conserved aspartates, glutamates and arginines in the active site region of Escherichia coli DNA topoisomerase I.

Zhu, C X; Roche, C J; Papanicolaou, N; DiPietrantonio, A; Tse-Dinh, Y C

Zhu, C X; Roche, C J; Papanicolaou, N; DiPietrantonio, A; Tse-Dinh, Y C.

Affiliation

Zhu CX; Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA.

J Biol Chem ; 273(15): 8783-9, 1998 Apr 10.

Article in En | MEDLINE | ID: mdl-9535856

Subject(s)

Arginine; Aspartic Acid; DNA Topoisomerases, Type I/chemistry; DNA Topoisomerases, Type I/metabolism; Escherichia coli/enzymology; Glutamic Acid; Amino Acid Sequence; Amino Acid Substitution; Binding Sites; DNA Primers; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Spectrometry, Fluorescence

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Collection: 01-internacional Database: MEDLINE Main subject: Arginine / Aspartic Acid / DNA Topoisomerases, Type I / Glutamic Acid / Escherichia coli Language: En Journal: J Biol Chem Year: 1998 Document type: Article Affiliation country: United States Country of publication: United States

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