Evaluation of different subcellular fractions of Vibrio cholerae O139 in protection to challenge in experimental cholera.
FEMS Immunol Med Microbiol
; 19(4): 323-9, 1997 Dec.
Article
in En
| MEDLINE
| ID: mdl-9537758
ABSTRACT
Various cellular fractions of Vibrio cholerae O139 were prepared and evaluated in the rabbit ileal loop model of experimental cholera for identification of the protective antigen(s) relevant for vaccine development. Lipopolysaccharides (LPS) and capsular polysaccharides (CPS) of O139 strains and its cell surface, membrane and cytosolic fractions were assayed for antibacterial immunity, whereas the cholera toxin was examined for antitoxic immunity. The lipopolysaccharides, membrane fraction and cholera toxin induced moderate protection, however there was a significant synergistic effect when cholera toxin was combined with membrane proteins or lipopolysaccharides. The O139 strains strongly resembled O1 strains in the profile of proteins and immunological cross reactivity, yet there was no cross protection. The results warrant further investigation of the pathogenesis of O139 strains and identify the critical somatic antigens relevant to protection.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vibrio cholerae
/
Cholera Vaccines
/
Cholera
Limits:
Animals
Language:
En
Journal:
FEMS Immunol Med Microbiol
Journal subject:
ALERGIA E IMUNOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Year:
1997
Document type:
Article
Affiliation country:
India