The yhhP gene encoding a small ubiquitous protein is fundamental for normal cell growth of Escherichia coli.
J Bacteriol
; 180(8): 2257-61, 1998 Apr.
Article
in En
| MEDLINE
| ID: mdl-9555915
ABSTRACT
H-NS is a major constituent of the Escherichia coli nucleoid, whereas sigmaS is a stress-induced sigma factor. An hns null mutation affects the cellular content of sigmaS in such a way that a remarkable accumulation of sigmaS is observed in the logarithmic growth phase, which results in enhanced expression of a number of sigmaS-dependent genes, including the katE gene. We isolated an extragenic mutation that affects the expression of the katE-lacZ fusion gene in the deltahns background. The relevant gene was identified as yhhP, which encodes a small polypeptide of 81 amino acids. Lesion of this gene seemed to affect the stability of sigmaS. A deletion analysis of yhhP revealed that this small protein plays a fundamental role in the general physiology of E. coli. The yhhP-deficient cell is not capable of growing in standard laboratory rich medium (i.e., Luria broth), resulting in the formation of filamentous cells. Homologs of this intriguing protein occur in a wide variety of bacterial species, including archaeal species.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Escherichia coli Proteins
/
Escherichia coli
Type of study:
Prognostic_studies
Language:
En
Journal:
J Bacteriol
Year:
1998
Document type:
Article
Affiliation country:
Japan