In vitro and in vivo comparison of a randomly coupled antibody fragment-enzyme conjugate with a site-specific conjugate.
Biomed Pept Proteins Nucleic Acids
; 1(5): 251-4, 1995.
Article
in En
| MEDLINE
| ID: mdl-9575348
ABSTRACT
Two antibody fragment-enzyme conjugates, one obtained by random coupling of the two protein component, the other by site-specific ligation of the same component, were compared in vitro and in vivo for their usefulness in antibody directed enzyme prodrug therapy (ADEPT). The in vitro studies have shown that the site-specific conjugate has a higher antigen binding capacity, while both conjugates had similar specific enzymic activities. In vivo, the site-specific conjugate was cleared more rapidly. When correction was made for this faster clearance, both conjugates showed similar antitumor efficacy in a mouse xenograft system upon administration of a prodrug.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Immunoglobulin Fragments
/
Gamma-Glutamyl Hydrolase
/
Immunoconjugates
Type of study:
Clinical_trials
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
Biomed Pept Proteins Nucleic Acids
Journal subject:
BIOQUIMICA
Year:
1995
Document type:
Article
Affiliation country:
Switzerland