Molecular characterization of the gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae.
J Bacteriol
; 180(11): 2854-61, 1998 Jun.
Article
in En
| MEDLINE
| ID: mdl-9603872
ABSTRACT
The gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae was cloned and sequenced. The gyrA gene codes for a protein of 822 amino acids homologous to the gyrase A subunit of eubacteria. Translation of the gene in an Escherichia coli expression system revealed a 92-kDa polypeptide. A sequence-directed DNA curvature was identified in the promoter region of gyrA. The bend center was mapped and located between the -35 and -10 regions of the promoter. Primer extension analysis showed that gyrA transcription initiates 6 bp downstream of an extended -10 promoter. The possible implications of the bent DNA region as a regulatory element in the transcription of gyrA are discussed.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Streptococcus pneumoniae
/
DNA Topoisomerases, Type II
/
Genes, Bacterial
Language:
En
Journal:
J Bacteriol
Year:
1998
Document type:
Article
Affiliation country:
Spain