Mutation analysis of the c-mos proto-oncogene in human ovarian teratomas.
Br J Cancer
; 77(10): 1642-4, 1998 May.
Article
in En
| MEDLINE
| ID: mdl-9635841
ABSTRACT
Female transgenic mice lacking a functional c-mos proto-oncogene develop ovarian teratomas, indicating that c-mos may behave as a tumour-suppressor gene for this type of tumour. We have analysed the entire coding region of the c-MOS gene in a series of human ovarian teratomas to determine whether there are any cancer-causing alterations. DNA from twenty teratomas was analysed by single-strand conformational analysis (SSCA) and heteroduplex analysis (HA) to screen for somatic and germline mutations. In nine of these tumours the entire gene was also sequenced. A previously reported polymorphism and a single new sequence variant were identified, neither of which we would predict to be disease-causing alterations. These results suggest that mutations in the coding region of the c-MOS gene do not play a significant role in the genesis of human ovarian teratomas.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ovarian Neoplasms
/
Teratoma
/
Genes, mos
Limits:
Female
/
Humans
Language:
En
Journal:
Br J Cancer
Year:
1998
Document type:
Article
Affiliation country:
United kingdom