5'-nucleotidase activity in lymphocytes from patients affected by B-cell chronic lymphocytic leukemia.
Clin Biochem
; 31(4): 269-72, 1998 Jun.
Article
in En
| MEDLINE
| ID: mdl-9646951
ABSTRACT
OBJECTIVES:
The activity of membrane-bound ecto-5'-nucleotidase and soluble e-Ns and c-N-II 5'-nucleotidases was evaluated on lymphocytes from patients affected by B-cell chronic lymphocytic leukemia (B-CLL). A statistically significative decrease in ecto-5'-nucleotidase, e-Ns, and c-N-II activities was observed in peripheral blood lymphocytes and in B and T populations from affected individuals. DESIGN ANDMETHODS:
For the assay of ecto-5'-nucleotidase, e-Ns, and c-N-II activity we used a radioactive procedure coupled to HPLC. Since the ecto-5'-nucleotidase is identified as CD73 antigen, we performed immunofluorescence analysis using a specific monoclonal antibody. We analyzed ecto-5'-nucleotidase mRNA by RT-PCR to ascertain the possibility of an alteration in the transcription of its gene.RESULTS:
A decrease in ecto-5'-nucleotidase activity was correlated to reduction in ecto-5'-nucleotidase positive cells (CD73+) in leukemia patients. RT-PCR produced a fragment of the expected size and the specific mRNA was found expressed in both healthy subjects and leukemia patients.CONCLUSIONS:
The decrease in ecto-5'-nucleotidase activity in patients with B-CLL is not due to loss of transcription of the specific mRNA. The presence of point mutations, splicing alteration, or posttranslational modifications must be investigated. If a defect at DNA or RNA level will be detected, the molecular analysis will be considered for diagnosis of B-cell chronic lymphocytic leukemia.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lymphocytes
/
Leukemia, Lymphocytic, Chronic, B-Cell
/
5'-Nucleotidase
Limits:
Aged
/
Humans
/
Middle aged
Language:
En
Journal:
Clin Biochem
Year:
1998
Document type:
Article
Affiliation country:
Italy