Immunohistochemical localization of ecdysteroid receptor and ultraspiracle in the epithelial cell line from Chironomus tentans (Insecta, Diptera).
Tissue Cell
; 30(2): 187-94, 1998 Apr.
Article
in En
| MEDLINE
| ID: mdl-9661293
ABSTRACT
Ecdysteroid receptor (EcR) and its heterodimerization partner, ultraspiracle (USP), were demonstrated in the epithelial cell line from Chironomus tentans by immunohistochemistry. In untreated cells both proteins are present in nuclei as well as in granular compartments of the cytosol. At 1 day after addition of 1-microM 20-OH-ecdysone (20E) total immunofluorescence had increased in the nuclei, whereas the cytoplasmic staining had disappeared. At the 2nd and 3rd days all cells within a vesicle appear identical according to morphological criteria, but the EcR and USP immunoreactivity becomes restricted into patches of neighbouring cells. The hormonally induced changes in the pattern of localization of functional ecdysteroid receptor, the heterodimer of EcR and USP, are discussed in relation to similar effects of 20E on acetylcholinesterase and muscarinic acetylcholine receptor distribution in this cell line.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Receptors, Steroid
/
Chironomidae
/
DNA-Binding Proteins
/
Epithelial Cells
Limits:
Animals
Language:
En
Journal:
Tissue Cell
Year:
1998
Document type:
Article
Affiliation country:
Germany