A viral mechanism for inhibition of the cellular phosphatase calcineurin.

Miskin, J E; Abrams, C C; Goatley, L C; Dixon, L K

Miskin, J E; Abrams, C C; Goatley, L C; Dixon, L K.

Affiliation

Miskin JE; Institute for Animal Health, Pirbright Laboratory, Pirbright, Surrey, GU24 0NF, UK.

Science ; 281(5376): 562-5, 1998 Jul 24.

Article in En | MEDLINE | ID: mdl-9677199

ABSTRACT

ABSTRACT

The transcription factor NFAT (nuclear factor of activated T cells) controls the expression of many immunomodulatory proteins. African swine fever virus inhibits proinflammatory cytokine expression in infected macrophages, and a viral protein A238L was found to display the activity of the immunosuppressive drug cyclosporin A by inhibiting NFAT-regulated gene transcription in vivo. This it does by binding the catalytic subunit of calcineurin and inhibiting calcineurin phosphatase activity.

Subject(s)

African Swine Fever Virus/physiology; Calcineurin Inhibitors; DNA-Binding Proteins/metabolism; Macrophages, Alveolar/virology; Nuclear Proteins; Transcription Factors/metabolism; Transcription, Genetic; Viral Proteins/metabolism; Amino Acid Sequence; Animals; Binding Sites; Calcineurin/metabolism; Cell Nucleus/metabolism; Cells, Cultured; Chlorocebus aethiops; Cyclosporine/pharmacology; DNA-Binding Proteins/genetics; Genes, Reporter; Molecular Sequence Data; NF-kappa B/metabolism; NFATC Transcription Factors; Recombinant Proteins/metabolism; Swine; Transcription Factors/genetics; Vero Cells; Viral Proteins/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / Transcription, Genetic / Viral Proteins / Nuclear Proteins / Macrophages, Alveolar / African Swine Fever Virus / DNA-Binding Proteins / Calcineurin Inhibitors Limits: Animals Language: En Journal: Science Year: 1998 Document type: Article Affiliation country: United kingdom

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